簡(jiǎn)要描述:英文名稱(chēng): WNT-5a, Human產(chǎn)品應(yīng)用: 信號(hào)表達(dá),胚胎發(fā)育,細(xì)胞增殖中國(guó)俗稱(chēng): 人WNT-5a 生長(zhǎng)分化因子 注冊(cè)品牌: StemRD 生產(chǎn)廠家: 美國(guó)StemRD公司訂購(gòu)貨號(hào): W5A-H-100規(guī)格型號(hào): 100?g保存溫度: ?-80℃產(chǎn)品種類(lèi): 生長(zhǎng)分化因子到貨時(shí)間: 1~5天
產(chǎn)品目錄
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產(chǎn)品英文名稱(chēng): WNT-5a, Human
產(chǎn)品中文名稱(chēng):人重組WNT-5a蛋白
訂購(gòu):
WNT-5a, Human產(chǎn)品介紹:
Wnt信號(hào)轉(zhuǎn)導(dǎo)通路因其啟動(dòng)蛋白為Wnt蛋白而得名,在胚胎發(fā)育、細(xì)胞增殖及分化等方面發(fā)揮重要 作用,其異?;罨c人類(lèi)多種腫瘤的發(fā)生密切相關(guān)。研究顯示,Wnt信號(hào)至少存在經(jīng)典和非經(jīng)典兩條不同的信號(hào)通路,而Wnt-5a屬于非經(jīng)典Wnt信號(hào)通 路,既往研究Ror2具有Wnt受體結(jié)構(gòu)域樣結(jié)構(gòu)。有研究認(rèn)為Wnt-5a經(jīng)由Ror2介導(dǎo)的 信號(hào)通路發(fā)揮作用。
Human WNT-5a蛋白含有357個(gè)氨基酸殘基。
WNT-5a, Human 產(chǎn)品特性:
? 活性在共轉(zhuǎn)Frizzled- 4 和 LRP- 5基因的HEK293 細(xì)胞鑒定,純度高,無(wú)動(dòng)物成分污染
? WNT-5a, Human活性在共轉(zhuǎn)Frizzled- 4 和 LRP- 5基因的HEK293 細(xì)胞鑒定
? WNT-5a, Human產(chǎn)品經(jīng)過(guò)SDS-PAGE 和HPLC雙層鑒定純度大于85%
WNT-5a, Human產(chǎn)品英文簡(jiǎn)述:
WNT-5a belongs to the class of WNT proteins that activate the “noncanonical" pathway. The predicted size of human WNT-5a is a monomeric protein containing 357 amino acid residues. Due to glycosylation, it migrates at an apparent molecular weight of ~45 kDa on SDS-PAGE under non-reducing conditions. StemRD’s product is expressed from a human cell line in animal-free medium, and purified with a proprietary process that is distinct from the published method.
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? Purity: Greater than 85% as determined by SDS-PAGE and HPLC analysis
? Biological Activity: The activity was determined by using a TCF reporter gene assay in HEK293 cells co-transfected with Frizzled-4 and LRP- 5. WNT-5a activates (instead of inhibits) the TCF reporter gene in this assay (MilkelsAJ, et al., PLoS Biol, 4:e115, 2006). This activation mode is utilized because activation assays are generally more reliable than inhibition assays, as they are less prone to any non-specific inhibitory contamination in the preparation.
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以下是*部分產(chǎn)品,更多請(qǐng)關(guān)注 www.stemcell.so
StemRD MGro-500 MSC medium,chemically-defined,間充質(zhì)干細(xì)胞培養(yǎng)基 500mL/瓶
LONZA 04-418Q X-VIVO 15 無(wú)血清培養(yǎng)基 1L/瓶
Life Technology 087-0112DK AIM-V? CTS? 無(wú)血清細(xì)胞培養(yǎng)基(細(xì)胞治療級(jí)) 1000mL/瓶
Pall 15950-017 Ultroser Serum Substitute 血清替代品 20mL/瓶
LONZA 12-725F UltraCULTURE Serum-free Medium無(wú)血清培養(yǎng)基 500mL/瓶
BD 762165 PAXgene Blood RNA Tube RNA樣本收集管 100管/盒
LONZA 50150 SeaKem? Gold Agarose 金瓊脂糖粉 125g/瓶
LONZA CC-3162 EGM-2 BulletKit( CC-3156 & CC-4176 )套裝 Kit/套
Nunc 159910 175cm2易用培養(yǎng)瓶,PS,已滅菌,瓶蓋:過(guò)濾蓋 30個(gè)/箱
LONZA LT07-318 MycoAlert? Mycoplasma Detection Kit支原體檢測(cè)試劑盒 100Tests/盒
LONZA LT07-418 MycoAlert? Mycoplasma Detection Kit支原體檢測(cè)試劑盒 50Tests/盒
Lonza 50-650U Kinetic-QCL 192 Test Kit內(nèi)毒素檢測(cè)試劑盒 192T/盒
Corning 431080 175cm⒉細(xì)胞培養(yǎng)瓶,Angled頸,透氣蓋 50個(gè)/箱
Corning 8388 125mL儲(chǔ)液瓶 Storage Bottles,PS,45mm瓶頸,帶蓋,滅菌,獨(dú)立包裝 24個(gè)/箱
Corning 430659 2ml凍存管, 聚丙烯,外旋蓋, 可自立圓底 500個(gè)/箱
Corning 3290 75cm2 CellBIND Surface培養(yǎng)瓶,已滅菌,透氣蓋 100個(gè)/箱
Corning 3292 175cm2 CellBIND Surface培養(yǎng)瓶,已滅菌,透氣蓋 50個(gè)/箱
BD-Falcon 352360 Cell Strainer, 100μm, Yellow 100um細(xì)胞濾網(wǎng) 50個(gè)/箱
BD Falcon 352070 50mL錐形離心管,高透明度聚丙烯,袋裝 500支/箱
BD Falcon 352096 15mL錐形離心管,高透明度聚丙烯,袋裝 500支/箱
BD BioCoat 354234 Matrigel Basement Membrane Matrix(標(biāo)準(zhǔn)型) 10mL/瓶
Cygnus F550 CHO HCP ELISA Kit,3G宿主蛋白檢測(cè)試劑盒 96T/盒
Cygnus F410 E.coli HCP ELISA Kit 大腸桿菌 96T/盒
Nalgene 5100-0001 Cryo 1℃ Freezing Container梯度降溫凍存盒 1個(gè)/箱
Nalgene 5026-0909 CryoBoxes 凍存盒 9X9 陣列,管瓶1.2/2.0mL 24個(gè)/箱
Nalgene 2250-0130 50L 可高溫高壓操作的細(xì)口大瓶(帶手柄) 1個(gè)/箱
Nalgene 2319-0050 20L 可高溫高壓滅菌的細(xì)口大瓶(帶放水口) 1個(gè)/箱
Hyclone SH30084.03 FBS 優(yōu)等澳洲胎牛血清 500mL/瓶
PeproTech 100-18B-500 Recombinant Human FGF-basic (154 a.a.)因子 500ug/支
Parafilm PM-996 Parafilm? M Laboratory Film 封口膜 12卷/箱